Part:BBa_M45424:Experience
Contribution of OUC-China
Group: iGEM Team OUC-China 2023
Author: Qingyu Wu
Summary: Exploring the expression conditions of the protein
In order to explore the induction conditions of this protein, we set different concentrations of IPTG and analyzed the SDS-PAGE results using software. Finally, we obtained the following table, which shows that there is not much difference in protein expression levels at three different concentrations, but the optimal concentration is 0.5mM.
Fig 1 Expression intensity of proteins at different induction concentrations
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M45424
User Reviews
UNIQ8cbb50cab2f275ce-partinfo-00000001-QINU UNIQ8cbb50cab2f275ce-partinfo-00000002-QINU